ANalysis Of Translational Activity (anota)
نویسندگان
چکیده
Translational control of gene expression is a mechanism that affects the relationship between mRNA level and protein level. Translation is commonly deregulated in human diseases such as cancer and understanding which mRNAs are targeted for translational deregulation and the mechanisms that mediate such effects is of high importance. Estimates of global translational activity has primarily been generated using the poly(ribo)some microarray approach (reviewed in [1]) but sequencing based methods have also recently been developed [2]. Both approaches are based on a parallel quantification of cytosolic mRNA level and the level of those mRNAs that are actively utilized for translation. During poly(ribo)some preparations, the cytosolic mRNA is isolated and separated based on the number of associated ribosomes. Fractions of mRNAs associated with several ribosomes are pooled and designated the translationally active pool. A parallel cytosolic mRNA sample, which is used to assess the cytosolic mRNA level, is also collected. More details are found in [1]. The recent sequencing
منابع مشابه
Anota: Analysis of Differential Translation in Genome-wide Studies
UNLABELLED Translational control of gene expression has emerged as a major mechanism that regulates many biological processes and shows dysregulation in human diseases including cancer. When studying differential translation, levels of both actively translating mRNAs and total cytosolic mRNAs are obtained where the latter is used to correct for a possible contribution of differential cytosolic ...
متن کاملPackage 'anota'
Maintainer Ola Larsson Description Genome wide studies of translational control is emerging as a tool to study verious biological conditions. The output from such analysis is both the mRNA level (e.g. cytosolic mRNA level) and the levl of mRNA actively involved in translation (the actively translating mRNA level) for each mRNA. The standard analysis ...
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